fluorescence recovery after photobleaching
- fluorescence recovery after photobleaching
-
(= FRAP)
Many
fluorochromes are bleached by exposure to exciting light. If, for example, the
cell surface is labelled with
a fluorescent
probe and an area bleached by laser illumination, then the bleached patch that starts off as
a dark area will gradually recover fluorescence. The recovery is due to the re-population of the area by unbleached molecules and diffusion of bleached molecules to other areas. The rate and extent of recovery are
a measure of the fluidity of the
membrane and the proportion of labelled molecules that are free to exchange with adjacent areas. The technique is usually applied to
cell surface fluidity or viscosity measurements, but is also applicable to other structures.
Dictionary of molecular biology.
2004.
Look at other dictionaries:
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Redistribution de fluorescence après photoblanchiment — La redistribution de fluorescence après photoblanchiment (FRAP, fluorescence recovery after photobleaching) est une méthode utilisée en microscopie à fluorescence pour mesurer la vitesse de diffusion moléculaire. Elle peut être appliquée pour… … Wikipédia en Français
FRAP — fluorescence recovery after photobleaching … Medical dictionary
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